Fig 1: HDAC1 modulates OGG1 activity.a, b Representative images and quantification of OGG1 acetylation assessed by western blot analysis using antibody against acetylated lysine (Ac-Lysine, 4G12). Recombinant OGG1 (100 ng) was first acetylated by p300 (25 ng), and then incubated with titrated amount of HDAC1 (25 and 50 ng). a, b Four replicates from four independent experiments. c Reaction scheme of OGG1 cleavage assay. 5'IRDye800CW-labeled oligonucleotides (33 nt) that contain an 8-oxoG base at position 17 and complementary unlabeled strand were used as OGG1 substrate. Recombinant OGG1 proteins were incubated with OGG1 substrate and resolved by 20% denaturing urea PAGE. d, e Representative images and quantification of OGG1 cleavage activity on the reaction samples shown in Fig. 3a. d, e Seven replicates from four independent experiments. Nuclear extracts from HEK cells overexpressing OGG1 were used as a positive control. f Co-immunoprecipitation of OGG1 with HDAC1 in cortical nuclear extracts from 2-month-old wild-type mice (n = 2) from one experiment. The asterisks indicate the nonspecific cross-reaction with the IgG heavy and light chains. g, h Representative images and quantification of acetylation status of endogenous OGG1 in hippocampal lysates of 13M control (n = 4) and Hdac1 cKO (n = 4) animals. g, h Two independent experiments. i Representative images of OGG1 cleavage assay using hippocampal nuclear extracts of 13M control and Hdac1 cKO mice. j Quantification of cleaved 17-nt products after OGG1 cleavage assay using hippocampal nuclear extracts of 13M control (n = 6) and Hdac1 cKO (n = 4) animals. i, j Two independent experiments. All values are shown as mean ± SEM. Statistical analysis: b, e one-way ANOVA with Tukey’s post hoc test; h, j two-tailed Student’s t test. Source data are provided as a Source Data file.